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addgene addgene plasmid  (Addgene inc)


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    Structured Review

    Addgene inc addgene addgene plasmid
    Addgene Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+shrna/pLKO-Tet-On-shRNA-Control+(Plasmid+%2398398)/pm42771527-264-18-18
    Average 93 stars, based on 54 article reviews
    addgene addgene plasmid - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Knockdown:

    Article Title: Biallelic MED29 variants cause pontocerebellar hypoplasia with cataracts.
    Article Snippet: .. For knockdown experiments, cotransfection was performed of the pool of Med29 shRNAs or the control shRNA with an RFP plasmid (Addgene). ..

    Article Title: Biallelic MED29 variants cause pontocerebellar hypoplasia with cataracts
    Article Snippet: .. For knockdown experiments, co-transfection was performed of the pool of Med29 shRNAs or the control shRNA with an RFP plasmid (Addgene). ..

    Cotransfection:

    Article Title: Biallelic MED29 variants cause pontocerebellar hypoplasia with cataracts.
    Article Snippet: .. For knockdown experiments, cotransfection was performed of the pool of Med29 shRNAs or the control shRNA with an RFP plasmid (Addgene). ..

    Control:

    Article Title: Biallelic MED29 variants cause pontocerebellar hypoplasia with cataracts.
    Article Snippet: .. For knockdown experiments, cotransfection was performed of the pool of Med29 shRNAs or the control shRNA with an RFP plasmid (Addgene). ..

    Article Title: GATAD2B O-GlcNAcylation Regulates Breast Cancer Stem-like Potential and Drug Resistance
    Article Snippet: .. Control shRNA (plasmid 1864) was purchased from Addgene. .. OGT shRNA was obtained from Sigma-Aldrich.

    Article Title: Biallelic MED29 variants cause pontocerebellar hypoplasia with cataracts
    Article Snippet: .. For knockdown experiments, co-transfection was performed of the pool of Med29 shRNAs or the control shRNA with an RFP plasmid (Addgene). ..

    Article Title: Investigating the Associations Between Hmga2 Overexpression, R-Loop Reduction, and Bone Loss in Aging Mice
    Article Snippet: .. The control shRNA (Consh, Houston, TX, USA) was purchased from Addgene (Cambridge, MA, USA). ..

    Article Title: Investigating the Associations Between Hmga2 Overexpression, R-Loop Reduction, and Bone Loss in Aging Mice.
    Article Snippet: .. The control shRNA (Consh, Houston, TX, USA) was purchased from Addgene (Cambridge, MA, USA). ..

    Article Title: Cellular reprogramming to reverse aging and promote organ and tissue regeneration
    Article Snippet: Control shRNA comprised the sequence 5′-GTTCAGATGTGCGGCGAGT-3′ (plasmid #85741 from Addgene). mTET1 (Tet1 shRNA) comprised the sequence 5′-GCTCATGGAGACTAGGTTTGG-3′ (plasmid #85742 from Addgene). mTet2 (Tet2 shRNA) comprised the sequence 5′-GGATGTAAGTTTGCCAGAAGC-3′ (Plasmid #85743 from Addgene). .. Control shRNA comprised the sequence 5′-GTTCAGATGTGCGGCGAGT-3′ (plasmid #85741 from Addgene). mTET1 (Tet1 shRNA) comprised the sequence 5′-GCTCATGGAGACTAGGTTTGG-3′ (plasmid #85742 from Addgene). mTet2 (Tet2 shRNA) comprised the sequence 5′-GGATGTAAGTTTGCCAGAAGC-3′ (Plasmid #85743 from Addgene). ..

    Article Title: Modulation of RASD2 by miRNA-485-5p Drives Thyroid Cancer Progression and Metastasis.
    Article Snippet: .. Short hairpin RNA (shRNA) targeting RASD2 and scrambled control shRNA were cloned into the pLKO.1- puro lentiviral vector (Addgene, #8453). ..

    Article Title: Supporting Information for CITED2 is a Conserved Regulator of the Uterine-Placental Interface
    Article Snippet: .. A control shRNA that does not target any known mammalian gene, pLKO.1-shSCR (plasmid 1864), was obtained from Addgene. .. Lentiviral packaging vectors were obtained from Addgene and included pMDLg/pRRE (plasmid 12251), pRSVRev (plasmid 12253), and pMD2.G (plasmid 12259).

    shRNA:

    Article Title: Biallelic MED29 variants cause pontocerebellar hypoplasia with cataracts.
    Article Snippet: .. For knockdown experiments, cotransfection was performed of the pool of Med29 shRNAs or the control shRNA with an RFP plasmid (Addgene). ..

    Article Title: GATAD2B O-GlcNAcylation Regulates Breast Cancer Stem-like Potential and Drug Resistance
    Article Snippet: .. Control shRNA (plasmid 1864) was purchased from Addgene. .. OGT shRNA was obtained from Sigma-Aldrich.

    Article Title: Biallelic MED29 variants cause pontocerebellar hypoplasia with cataracts
    Article Snippet: .. For knockdown experiments, co-transfection was performed of the pool of Med29 shRNAs or the control shRNA with an RFP plasmid (Addgene). ..

    Article Title: Investigating the Associations Between Hmga2 Overexpression, R-Loop Reduction, and Bone Loss in Aging Mice
    Article Snippet: .. The control shRNA (Consh, Houston, TX, USA) was purchased from Addgene (Cambridge, MA, USA). ..

    Article Title: Investigating the Associations Between Hmga2 Overexpression, R-Loop Reduction, and Bone Loss in Aging Mice.
    Article Snippet: .. The control shRNA (Consh, Houston, TX, USA) was purchased from Addgene (Cambridge, MA, USA). ..

    Article Title: Cellular reprogramming to reverse aging and promote organ and tissue regeneration
    Article Snippet: Control shRNA comprised the sequence 5′-GTTCAGATGTGCGGCGAGT-3′ (plasmid #85741 from Addgene). mTET1 (Tet1 shRNA) comprised the sequence 5′-GCTCATGGAGACTAGGTTTGG-3′ (plasmid #85742 from Addgene). mTet2 (Tet2 shRNA) comprised the sequence 5′-GGATGTAAGTTTGCCAGAAGC-3′ (Plasmid #85743 from Addgene). .. Control shRNA comprised the sequence 5′-GTTCAGATGTGCGGCGAGT-3′ (plasmid #85741 from Addgene). mTET1 (Tet1 shRNA) comprised the sequence 5′-GCTCATGGAGACTAGGTTTGG-3′ (plasmid #85742 from Addgene). mTet2 (Tet2 shRNA) comprised the sequence 5′-GGATGTAAGTTTGCCAGAAGC-3′ (Plasmid #85743 from Addgene). ..

    Article Title: Modulation of RASD2 by miRNA-485-5p Drives Thyroid Cancer Progression and Metastasis.
    Article Snippet: .. Short hairpin RNA (shRNA) targeting RASD2 and scrambled control shRNA were cloned into the pLKO.1- puro lentiviral vector (Addgene, #8453). ..

    Article Title: Supporting Information for CITED2 is a Conserved Regulator of the Uterine-Placental Interface
    Article Snippet: .. A control shRNA that does not target any known mammalian gene, pLKO.1-shSCR (plasmid 1864), was obtained from Addgene. .. Lentiviral packaging vectors were obtained from Addgene and included pMDLg/pRRE (plasmid 12251), pRSVRev (plasmid 12253), and pMD2.G (plasmid 12259).

    Plasmid Preparation:

    Article Title: Biallelic MED29 variants cause pontocerebellar hypoplasia with cataracts.
    Article Snippet: .. For knockdown experiments, cotransfection was performed of the pool of Med29 shRNAs or the control shRNA with an RFP plasmid (Addgene). ..

    Article Title: GATAD2B O-GlcNAcylation Regulates Breast Cancer Stem-like Potential and Drug Resistance
    Article Snippet: .. Control shRNA (plasmid 1864) was purchased from Addgene. .. OGT shRNA was obtained from Sigma-Aldrich.

    Article Title: Biallelic MED29 variants cause pontocerebellar hypoplasia with cataracts
    Article Snippet: .. For knockdown experiments, co-transfection was performed of the pool of Med29 shRNAs or the control shRNA with an RFP plasmid (Addgene). ..

    Article Title: Cellular reprogramming to reverse aging and promote organ and tissue regeneration
    Article Snippet: Control shRNA comprised the sequence 5′-GTTCAGATGTGCGGCGAGT-3′ (plasmid #85741 from Addgene). mTET1 (Tet1 shRNA) comprised the sequence 5′-GCTCATGGAGACTAGGTTTGG-3′ (plasmid #85742 from Addgene). mTet2 (Tet2 shRNA) comprised the sequence 5′-GGATGTAAGTTTGCCAGAAGC-3′ (Plasmid #85743 from Addgene). .. Control shRNA comprised the sequence 5′-GTTCAGATGTGCGGCGAGT-3′ (plasmid #85741 from Addgene). mTET1 (Tet1 shRNA) comprised the sequence 5′-GCTCATGGAGACTAGGTTTGG-3′ (plasmid #85742 from Addgene). mTet2 (Tet2 shRNA) comprised the sequence 5′-GGATGTAAGTTTGCCAGAAGC-3′ (Plasmid #85743 from Addgene). ..

    Article Title: Supporting Information for CITED2 is a Conserved Regulator of the Uterine-Placental Interface
    Article Snippet: .. A control shRNA that does not target any known mammalian gene, pLKO.1-shSCR (plasmid 1864), was obtained from Addgene. .. Lentiviral packaging vectors were obtained from Addgene and included pMDLg/pRRE (plasmid 12251), pRSVRev (plasmid 12253), and pMD2.G (plasmid 12259).

    Sequencing:

    Article Title: Cellular reprogramming to reverse aging and promote organ and tissue regeneration
    Article Snippet: Control shRNA comprised the sequence 5′-GTTCAGATGTGCGGCGAGT-3′ (plasmid #85741 from Addgene). mTET1 (Tet1 shRNA) comprised the sequence 5′-GCTCATGGAGACTAGGTTTGG-3′ (plasmid #85742 from Addgene). mTet2 (Tet2 shRNA) comprised the sequence 5′-GGATGTAAGTTTGCCAGAAGC-3′ (Plasmid #85743 from Addgene). .. Control shRNA comprised the sequence 5′-GTTCAGATGTGCGGCGAGT-3′ (plasmid #85741 from Addgene). mTET1 (Tet1 shRNA) comprised the sequence 5′-GCTCATGGAGACTAGGTTTGG-3′ (plasmid #85742 from Addgene). mTet2 (Tet2 shRNA) comprised the sequence 5′-GGATGTAAGTTTGCCAGAAGC-3′ (Plasmid #85743 from Addgene). ..

    Clone Assay:

    Article Title: Modulation of RASD2 by miRNA-485-5p Drives Thyroid Cancer Progression and Metastasis.
    Article Snippet: .. Short hairpin RNA (shRNA) targeting RASD2 and scrambled control shRNA were cloned into the pLKO.1- puro lentiviral vector (Addgene, #8453). ..



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    Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by <t>shRNA.</t> ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.
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    Image Search Results


    Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by shRNA. ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.

    Journal: American Journal of Respiratory Cell and Molecular Biology

    Article Title: Piezo1 mediates stretch-induced Wnt/β-catenin signaling and extracellular matrix remodeling in aging asthmatic airway smooth muscle

    doi: 10.1093/ajrcmb/aanag058

    Figure Lengend Snippet: Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by shRNA. ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.

    Article Snippet: Control shRNA lentiviral particles-A (sc-108080) and Pz1 shRNA lentiviral particles (sc-93227-V) were procured from Santa Cruz Biotechnology (TX, USA).

    Techniques: CyQUANT Assay, Comparison, Expressing, shRNA, Knockdown, Western Blot, Analysis